Fractal analysis of resting state functional connectivity of the brain

A variety of resting state neuroimaging data tend to exhibit fractal behavior where its power spectrum follows power-law scaling. Resting state functional connectivity is significantly influenced by fractal behavior which may not directly originate from neuronal population activities of the brain. To describe the fractal behavior, we adopted the fractionally integrated process (FIP) model instead of the fractional Gaussian noise (FGN) since the FIP model covers more general aspects of fractality than the FGN model. We also introduce a novel concept called the nonfractal connectivity which is defined as the correlation of short memory independent of fractal behavior, and compared it with the fractal connectivity which is an asymptotic wavelet correlation. We propose several wavelet-based estimators of fractal connectivity and nonfractal connectivity for a multivariate fractionally integrated noise (mFIN). The performance of these estimators was evaluated through simulation studies and the analyses of resting state functional MRI data of the rat brain.Comment: The 2012 International Joint Conference on Neural Network

Player Perception of Delays and Jitter in Character Responsiveness

Response lag in digital games is known to negatively affect a player’s game experience. Particularly with networked multiplayer games, where lag is typically unavoidable, the impact of delays needs to be well understood so that its effects can be mitigated. In this paper, we investigate two aspects of lag independently: latency (constant delay) and jitter (varying delay). We evaluate how latency and jitter each affect a player’s enjoyment, frustration, performance, and experience as well as the extent to which players can adjust to such delays after a few minutes of gameplay. We focus on a platform game where the player controls a virtual character through a world. We find that delays up to 300ms do not impact the players’ experience as long as they are constant. When jitter was added to a delay of 200ms, however, the lag was noticed by participants more often, hindered players’ ability to improve with practice, increased how often they failed to reach the goal of the game, and reduced the perceived motion quality of the character

Dietary bioactive lipid compounds rich in menthol alter Interactions among members of ruminal microbiota in sheep

This study aimed to investigate the effects of two practically relevant doses of menthol-rich plant bioactive lipid compounds (PBLC) on fermentation, microbial community composition, and their interactions in sheep rumen. Twenty-four growing Suffolk sheep were divided into three treatments and were fed hay ad libitum plus 600 g/d of concentrate containing no PBLC (Control) or PBLC at low dose (80 mg/d; PBLC-L) or high dose (160 mg/d; PBLC-H). After 4 weeks on the diets, samples of ruminal digesta were collected and analyzed for short-chain fatty acid (SCFA), ammonia, and microbiota; microbiota being analyzed in the solid and the liquid digesta fractions separately. Ruminal SCFA and ammonia concentrations were not affected by the PBLC treatments. The microbiota in the solid fraction was more diverse than that in the liquid fraction, and the relative abundance of most taxa differed between these two fractions. In the solid fraction, phylogenetic diversity increased linearly with increased PBLC doses, whereas evenness (lowest in PBLC-L) and Simpson diversity index (greatest in PBLC-H) changed quadratically. In the liquid fraction, however, the PBLC supplementation did not affect any of the microbial diversity measurements. Among phyla, Chloroflexi (highest in PBLC-L) and unclassified_bacteria (lowest in PBLC-L) were altered quadratically by PBLC. Lachnospiraceae, Bacteroidaceae (increased linearly), BS11 (increased in PBLC-L), Christensenellaceae (decreased in PBLC treatments), and Porphyromonadaceae (increased in PBLC treatments) were affected at the family level. Among genera, Butyrivibrio increased linearly in the solid fraction, YRC22 increased linearly in the liquid fraction, whereas Paludibacter increased and BF311 increased linearly with increasing doses of PBLC in both fractions. The PBLC treatments also lowered methanogens within the classes Thermoplasmata and Euryarchaeota. Correlation network analysis revealed positive and negative correlations among many microbial taxa. Differential network analysis showed that PBLC supplementation changed the correlation between some microbial taxa and SCFA. The majority of the predicted functional features were different between the solid and the liquid digesta fractions, whereas the PBLC treatments altered few of the predicted functional gene categories. Overall, dietary PBLC treatments had little influence on the ruminal fermentation and microbiota but affected the associations among some microbial taxa and SCFA

Agrarvögel – ökologische Bewertungsgrundlage für Biodiversitätsziele in Ackerbaugebieten: Schlussfolgerungen für die Politikberatung

Agrarvögel sind wichtige Bioindikatoren für landwirtschaftliche Gebiete. Die Datenlage des Vogelindikators zeigt für die deutschen Agrargebiete aktuell eine negative Entwicklung von Artenvielfalt und Landschaftsqualität. Diese Sachlage erfordert für das Erreichen der Biodiversitätsziele dringend wirkungsvollere Naturschutz und Umweltmaßnahmen im Agrarbereich. Um dies zu erreichen, wurden Methoden zur Identifizierung der Lebensraumeignung von Indikatorvogelarten entwickelt, Wirkungen landwirtschaftlicher Maßnahmen auf Agrarvögel analysiert und Vorschläge für biodiversitätsfreundliche Nutzungssysteme abgeleitet. Forschungsprojekte und lokale Schutzmaßnahmen bilden erfolgreiche Elemente der Biodiversitätssicherung für die Agrargebiete. Für nachhaltige Nutzungssysteme mit Gewährleistung des Biodiversitätsschutzes werden jedoch, wie mit dem Greening der EU anvisiert, in allen Agrargebieten wirksame Naturschutzmaßnahmen unumgänglich. Hinreichende Vorteilswirkungen wären dafür durch ökologische Vorrangflächen erreichbar, wenn diese hohe Habitatqualitäten und ausreichenden Flächenumfang aufweisen. Im Sinne eines flexiblen Tools sollten Optionen der Auswahl betrieblich geeigneter Maßnahmen für den Landwirt bestehen, die jedoch in Abhängigkeit von der Habitatqualität eine Flächengewichtung ökologischer Vorrangflächen erfordern. Zudem wird ein gezieltes Management dieser Nutzflächen im landwirtschaftlichen Betrieb erforderlich.Stichwörter: Agrarvögel, Bioindikatoren, landwirtschaftliche Gebiete, Biodiversitätsziele, ökologische Vorrangflächen, Habitatqualitäten, Management.Farmland Birds - ecological basis for the evaluation of biodiversity targets in agricultural lands: conclusions for policy adviceAbstractFarmland birds act as important bio-indicators for agricultural areas. Bird-based indicators reveal current negative trends in species diversity and landscape quality of German agricultural areas. Urgent, more effective nature protection and environmental measures are needed in agricultural areas to reach biodiversity targets. To this end, methods were developed to assess habitat suitability of indicator bird species, impacts of agricultural measures on farmland birds were analyzed and suggestions for a biodiversity-friendly land-use were derived. Research projects and local conservation measures are effective tools of biodiversity conservation in agricultural areas. Sustainable land-use forms that ensure biodiversity preservation inevitably need efficient nature protection measures in agricultural areas, as aimed by the EU’s Greening policy. Sufficiently beneficial effects could be reached through ecological focus areas, where these hold high habitat qualities and are large enough. Farmers should be offered a choice of nature protection measures from which they can select the most operationally applicable to their situation. This, however, requires a weighting of measures of the ecological focus areas as a function of habitat quality. Also, a specific management way is necessary for this agricultural land.Keywords: Farmland birds, bio-indicators, agricultural areas, biodiversity aims, ecological focus areas, habitat quality, management

Dietary supplementation of menthol-rich bioactive lipid compounds alters circadian eating behaviour of sheep

Background: Plant bioactive lipid compounds (PBLC), commonly known as essential oils, are increasingly evaluated as feed additives in ruminants due to beneficial effects on animal performance and health; however, there is no study evaluating circadian eating behaviour in ruminants. Altered eating behaviour may be implicated in changes of feed intake in ruminants. Therefore, the present study investigated the influence of menthol-rich PBLC on circadian eating behaviour in 24 growing sheep that were equally divided into three treatments, control (without PBLC), a lower dose (80 mg/d) or a higher dose (160 mg/d) of PBLC. Daily doses of PBLC were supplied with 600 g/d of concentrates fed in three equal portions at 07:00, 11:00 and 15:00 h for 4 weeks, whereas, meadow hay was fed ad libitum. Results: The eating behaviour recorded by an automatic transponder-operated feeding system revealed that daily eating time and feeder visits increased with increasing doses of PBLC. The circadian distribution of eating time and feeder visits (with 1-h resolution) was influenced by the treatment. Eating time during concentrate-offering hours and between concentrate-offering hours increased or tended to increase linearly with greater concentrations of PBLC. Feeder visits did not change significantly during concentrate-offering hours, but were greater in the PBLC groups compared with the control between concentrate-feeding hours. Average length of the longest meals (5th percentile) decreased due to PBLC feeding. Daily feed intake was greater in the PBLC groups than the control. Conclusions: Menthol-rich PBLC in the applied dose range stimulate circadian eating behaviour, which cannot only be attributed to their presence during concentrate feeding hours, but persist during post-concentrate feeding hours

Effects of dietary menthol-rich bioactive lipid compounds on zootechnical traits, blood variables and gastrointestinal function in growing sheep

Background The present study aimed at investigating the influence of 90% menthol-containing plant bioactive lipid compounds (PBLC, essential oils) on growth performance, blood haematological and biochemical profile, and nutrient absorption in sheep. Twenty-four growing Suffolk sheep were allotted into three dietary treatments: Control (without PBLC), lower dose of PBLC (PBLC-L; 80 mg/d) and higher dose of PBLC (PBLC-H; 160 mg/d). Sheep in all groups were fed meadow hay ad libitum plus 600 g/d of concentrate pellets for 28 d. Results Average daily gain was not affected by treatment. Feeding of PBLC increased hay and total feed intake per kg body weight (P < 0.05). Counts of total leucocytes, lymphocytes and monocytes were not different among treatments. However, neutrophil count decreased (P < 0.05) in PBLC-H with a similar trend in PBLC-L (P < 0.10). Concentrations of glucose, bilirubin, triglycerides, cholesterol, urea and magnesium in serum were not different among sheep fed different doses of PBLC. However, serum calcium concentration tended to increase in PBLC-H (P < 0.10) and serum concentrations of aspartate & asparagine (P < 0.01) and glutamate & glutamine (P < 0.05) increased linearly with increasing PBLC dose. In ruminal epithelia isolated from the rumen after killing, baseline conductance (Gt; P < 0.05) and short-circuit current (Isc; P < 0.01) increased in both PBLC groups. Ruminal uptakes of glucose and methionine in the presence of Na+ were not affected by the dietary PBLC supplementation. In the absence of Na+, however, glucose and methionine uptakes increased (P < 0.05) in PBLC-H. In the jejunum, Isc tended to increase in PBLC-H (P < 0.10), but baseline Gt was not affected. Intestinal uptakes of glucose and methionine were not influenced by PBLC in the presence or absence of Na+. Conclusion The results suggest that menthol-rich PBLC increase feed intake, and passive ion and nutrient transport, the latter specifically in the rumen. They also increased serum concentrations of urea precursor amino acids and tended to increase serum calcium concentrations. Future studies will have to show whether some of these findings might be commonly linked to a stimulation of transient receptor potential (TRP) channels in the gastrointestinal tract

Evaluating Grasping Visualizations and Control Modes in a VR Game

A primary goal of the Virtual Reality(VR) community is to build fully immersive and presence-inducing environments with seamless and natural interactions. To reach this goal, researchers are investigating how to best directly use our hands to interact with a virtual environment using hand tracking. Most studies in this field require participants to perform repetitive tasks. In this article, we investigate if results of such studies translate into a real application and game-like experience. We designed a virtual escape room in which participants interact with various objects to gather clues and complete puzzles. In a between-subjects study, we examine the effects of two input modalities (controllers vs. hand tracking) and two grasping visualizations (continuously tracked hands vs. virtual hands that disappear when grasping) on ownership, realism, efficiency, enjoyment, and presence. Our results show that ownership, realism, enjoyment, and presence increased when using hand tracking compared to controllers. Visualizing the tracked hands during grasps leads to higher ratings in one of our ownership questions and one of our enjoyment questions compared to having the virtual hands disappear during grasps as is common in many applications. We also confirm some of the main results of two studies that have a repetitive design in a more realistic gaming scenario that might be closer to a typical user experience

The GadX regulon affects virulence gene expression and adhesion of porcine enteropathogenic Escherichia coli in vitro

The ability of enteropathogenic Escherichia coli (EPEC) to express virulence factor genes and develop attaching and effacing (AE) lesions is inhibited in acidic environmental conditions. This inhibition is due to the activation of transcription factor GadX, which upregulates expression of glutamic acid decarboxylase (Gad). Gad, in turn, produces γ-aminobutyric acid (GABA), which was recently shown to have a beneficial effect on the jejunal epithelium in vitro due to increased mucin-1 levels. In the present study, we sought to test whether forced GadX activation/overexpression abolishes virulence associated features of EPEC and provokes increased GABA production. EPEC strains were isolated from diarrheic pigs and submitted to activation of GadX by acidification as well as gadX overexpression via an inducible expression vector plasmid. GABA concentrations in the growth medium, ability for adhesion to porcine intestinal epithelial cells (IPEC-J2) and virulence gene expression were determined. Growth in acidified media led to increased GABA levels, upregulated gadA/B expression and downregulated mRNA synthesis of the bacterial adhesin intimin. EPEC strains transformed with the gadX gene produced 2.1 to 3.4-fold higher GABA levels than empty-vector controls and completely lost their ability to adhere to IPEC-J2 cells and to induce actin accumulation. We conclude that intensified gadX activation can abolish the ability of EPEC to adhere to the intestinal epithelium by reducing the expression of major virulence genes

Matriderm® 1mm versus Integra® Single Layer 1.3mm for one-step closure of full thickness skin defects: a comparative experimental study in rats

Purpose: Dermal templates, such as Matriderm® and Integra®, are widely used in plastic and reconstructive surgery, often as two-step procedures. A recent development is the application of thin dermal templates covered with split thickness skin grafts in one-step procedures. In this experimental study, we compare the two thin matrices Matriderm® 1mm and Integra® Single Layer in a one-step procedure with particular focus on neodermis formation. Methods: Matriderm® 1mm and Integra® Dermal Regeneration Template—Single Layer (1.3mm) were compared in a rat model. In three groups of five animals each, a full thickness wound was covered with (a) Matriderm® 1mm and neonatal rat epidermis, (b) Integra® Single Layer and neonatal rat epidermis, or, (c) neonatal rat epidermis only (control). Histological sections 2weeks post transplantation were analyzed with regard to take of template and epidermis, neodermal thickness, collagen deposition, vascularization, and inflammatory response. Results: Take of both templates was complete in all animals. The Matriderm®-based neodermis was thinner but showed a higher cell density than the Integra®-based neodermis. The other parameters were similar in both matrices. Conclusion: The two templates demonstrate a comparable biological behavior early after transplantation. The only difference was found regarding neodermal thickness, probably resulting from faster degradation of Matriderm®. These preliminary data suggest that both dermal templates appear similarly suitable for transplantation in a one-step procedur